Separation in Hybridization

Evaluation of Discontinues Puresperm Gradient in Separation of X And Y Bearing Human Spermatozoa by Fluorescent in Situ Hybridization (FISH)

Introduction: To evaluate the 8 layer discontinues puresperm gradient in separation of human spermatozoa according to sex chromosomes by fluorescent in situ hybridization (FISH). Material and Methods: This study was carried out on three patient referring to Royan fertility and infertility center. Semen analysis was assessed according to WHO criteria. Each sample was divided into two aliquots, ...

Cell-specific photosynthetic gene expression in maize determined using cell separation techniques and hybridization in situ.

Bundle sheath strands and mesophyll cell extracts have been isolated from maize (Zea mays L.) leaves using a mechanical disruption-filtration technique. Northern blot analysis showed that phosphoenolpyruvate carboxylase (PEPCase; EC 4.1.1.31) mRNA accumulates only in mesophyll cells. The mechanisms regulating the cell-specific expression of this gene must, therefore, be at either the level of R...

Immunomagnetic separation and DNA hybridization for detection of enterotoxigenic Escherichia coli in a piglet model.

Enterotoxigenic Escherichia coli (ETEC) strains were detected by stool blot hybridization assays using different oligonucleotide probes for the colonization fimbrial antigen F4, heat-stable enterotoxin I (ST I), and heat-labile enterotoxin (LT I) genes. Forty-eight fecal samples and seven samples of intestinal content from ETEC-challenged newborn piglets were processed in two ways: (i) by direc...

Intraspecific Hybridization of Populus euphratica Oliv. Using in vitro Technique

Hybridization of a macroporous sponge and spherical microporous adsorbents for high throughput separation of ionic solutes.

Hybrid materials using a macroporous sponge and spherical microporous adsorbents have been developed for an effective rapid pretreatment of water samples. Various adsorbents, including methacrylate series, divinylbenzene (DVB), and graphite particles, were utilized for hybridization with a macroporous sponge consisting of polyethylene and polyvinyl acetate, EVA resin. Both the EVA resin and eac...